Enhancement of Salinity Stress Tolerance in Lettuce (Lactuca sativa L.) via Foliar Application of Nitric Oxide

Hasan Sardar (1) , Zubair Khalid (1) , Muhammad Ahsan (2) , Safina Naz (1) , Aamir Nawaz (1) , Riaz Ahmad (3) , Kashif Razzaq (4) , Saikh Wabaidur (5) , Cédric Jacquard (6) , Ivan Širić (7) , Pankaj Kumar , Sami Abou Fayssal (8)

1 BZU - Bahauddin Zakariya University
2 IUB - The Islamia University of Bahawalpur
3 Department of Biological Sciences [Dera Ismail Khan]
4 Muhammad Nawaz Shareef University of Agriculture
5 KSU - King Saud University [Riyadh]
6 RIBP - Résistance Induite et Bioprotection des Plantes - EA 4707
7 University of Zagreb
8 كلية الزراعة [الجامعة اللبنانية] = Faculty of Agronomy [Lebanese University] = Faculté d'Agronomie [Université libanaise]

Salt stress negatively affects the growth, development, and yield of horticultural crops. Nitric oxide (NO) is considered a signaling molecule that plays a key role in the plant defense system under salt stress. This study investigated the impact of exogenous application of 0.2 mM of sodium nitroprusside (SNP, an NO donor) on the salt tolerance and physiological and morphological characteristics of lettuce (Lactuca sativa L.) under salt stress (25, 50, 75, and 100 mM). Salt stress caused a marked decrease in growth, yield, carotenoids and photosynthetic pigments in stressed plants as compared to control ones. Results showed that salt stress significantly affected the oxidative compounds (superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX)) and non-oxidative compounds (ascorbic acid, total phenols, malondialdehyde (MDA), proline, and H2O2) in lettuce. Moreover, salt stress decreased nitrogen (N), phosphorous (P), and potassium ions (K+) while increasing Na ions (Na+) in the leaves of lettuce under salt stress. The exogenous application of NO increased ascorbic acid, total phenols, antioxidant enzymes (SOD, POD, CAT, and APX) and MDA content in the leaves of lettuce under salt stress. In addition, the exogenous application of NO decreased H2O2 content in plants under salt stress. Moreover, the exogenous application of NO increased leaf N in control, and leaf P and leaf and root K+ content in all treatments while decreasing leaf Na+ in salt-stressed lettuce plants. These results provide evidence that the exogenous application of NO on lettuce helps mitigate salt stress effects.

Domaines: Sciences de l'environnement

Hasan Sardar, Zubair Khalid, Muhammad Ahsan, Safina Naz, Aamir Nawaz, et al.. Enhancement of Salinity Stress Tolerance in Lettuce (Lactuca sativa L.) via Foliar Application of Nitric Oxide. Plants, 2023, 12 (5), pp.1115. ⟨10.3390/plants12051115⟩. ⟨hal-04335970⟩