Systemic modulation of stress and immune parameters in patients treated for prostate adenocarcinoma by intensity-modulated radiation therapy or stereotactic ablative body radiotherapy

B. Frey (1) , J. Mika (2) , K. Jelonek (3) , L. Cruz-Garcia (4) , C. Roelants (5) , I. Testard (6) , N. Cherradi (7) , K. Lumniczky (8) , S. Polozov (4, 9) , A. Napieralska (3) , P. Widlak (3) , U.S. Gaipl (1) , C. Badie (4) , J. Polanska (2) , S.M. Candéias (6)

1 Department of Radiation Oncology, University Hospital of Erlangen
2 Department of Data Science and Engineering,Silesian University of Technology
3 Maria Sklodowska-Curie National Research Institute of Oncology
4 Centre for Radiation, Chemical and Environmental Hazards
5 Inovarion [Paris]
6 ProMD - Protéomique, Métaux et Différenciation
7 IMAC - Invasion mechanisms in angiogenesis and cancer
8 National center for public health [Hungary]
9 HQ Science Limited

Background In this exploratory study, the impact of local irradiation on systemic changes in stress and immune parameters was investigated in eight patients treated with intensity-modulated radiation therapy (IMRT) or stereotactic ablative body radiotherapy (SABR) for prostate adenocarcinoma to gain deeper insights into how radiotherapy (RT) modulates the immune system. Patients and methods RT-qPCR, flow cytometry, metabolomics, and antibody arrays were used to monitor a panel of stress- and immune-related parameters before RT, after the first fraction (SABR) or the first week of treatment (IMRT), after the last fraction, and 3 weeks later in the blood of IMRT (N & x202f;= 4) or SABR (N & x202f;= 4) patients. Effect size analysis was used for comparison of results at different timepoints. Results Several parameters were found to be differentially modulated in IMRT and SABR patients: the expression ofTGFB1,IL1B, andCCL3genes; the expression of HLA-DR on circulating monocytes; the abundance and ratio of phosphatidylcholine and lysophosphatidylcholine metabolites in plasma. More immune modulators in plasma were modulated during IMRT than SABR, with only two common proteins, namely GDF-15 and Tim-3. Conclusion Locally delivered RT induces systemic modulation of the immune system in prostate adenocarcinoma patients. IMRT and SABR appear to specifically affect distinct immune components.

Mots clés: Ionizing radiation; Biomarkers of radiation exposure; Prostate cancer; Systemic immune modulation; Immunophenotyping

Domaines: Immunologie; Cancer

B. Frey, J. Mika, K. Jelonek, L. Cruz-Garcia, C. Roelants, et al.. Systemic modulation of stress and immune parameters in patients treated for prostate adenocarcinoma by intensity-modulated radiation therapy or stereotactic ablative body radiotherapy. Strahlentherapie und Onkologie, 2020, 196 (11), pp.‏ 1018-1033. ⟨10.1007/s00066-020-01637-5⟩. ⟨hal-02892711⟩