Dissecting the respective roles of microbiota and host genetics in the susceptibility of Card9−/− mice to colitis

Camille Danne (1, 2, 3) , Bruno Lamas (1, 2, 3) , Aonghus Lavelle (4) , Marie-Laure Michel (1, 3) , Gregory da Costa (1, 3) , Hang Phuong Pham (5) , Antoine Lefèvre (6, 7) , Chantal Bridonneau (1, 3) , Marius Bredon (2, 3) , Julien Planchais (1, 3) , Marjolene Straube (2, 3) , Patrick Emond (6, 7, 8) , Philippe Langella (1, 3) , Harry Sokol (2, 1, 3)

1 MICALIS - MICrobiologie de l'ALImentation au Service de la Santé
2 CRSA - Centre de Recherche Saint-Antoine
3 FHU PREMA - Fédération Hospitalo-Universitaire Combattre la prématurité
4 UCC - University College Cork
5 Parean biotechnologies
6 iBraiN - Imaging, Brain & Neuropsychiatry
7 UT - Université de Tours
8 Département de Pharmacologie [CHRU Tours]

Background The etiology of inflammatory bowel disease (IBD) is unclear but involves both genetics and environmental factors, including the gut microbiota. Indeed, exacerbated activation of the gastrointestinal immune system toward the gut microbiota occurs in genetically susceptible hosts and under the infuence of the environment. For instance, a majority of IBD susceptibility loci lie within genes involved in immune responses, such as caspase recruitment domain member 9 (Card9). However, the relative impacts of genotype versus microbiota on colitis susceptibility in the context of CARD9 defciency remain unknown. Results Card9 gene directly contributes to recovery from dextran sodium sulfate (DSS)-induced colitis by inducing the colonic expression of the cytokine IL-22 and the antimicrobial peptides Reg3β and Reg3γ independently of the microbiota. On the other hand, Card9 is required for regulating the microbiota capacity to produce AhR ligands, which leads to the production of IL-22 in the colon, promoting recovery after colitis. In addition, cross-fostering experiments showed that 5 weeks after weaning, the microbiota transmitted from the nursing mother before weaning had a stronger impact on the tryptophan metabolism of the pups than the pups’ own genotype. Conclusions These results show the role of CARD9 and its effector IL-22 in mediating recovery from DSS induced colitis in both microbiota-independent and microbiota-dependent manners. Card9 genotype modulates the microbiota metabolic capacity to produce AhR ligands, but this effect can be over ridden by the implantation of a WT or“healthy” microbiota before weaning. It highlights the importance of the weaning reaction occurring between the immune system and microbiota for host metabolism and immune functions through out life. A better understanding of the impact of genetics on microbiota metabolism is key to developing efficient therapeutic strategies for patients suffering from complex inflammatory disorders.

Keywords: Gut microbiota; Genetics; Metabolism; CARD9; IL-22; Trp metabolism; AhR ligands; Lactobacillus

Domains: Life Sciences [q-bio]

Camille Danne, Bruno Lamas, Aonghus Lavelle, Marie-Laure Michel, Gregory da Costa, et al.. Dissecting the respective roles of microbiota and host genetics in the susceptibility of Card9−/− mice to colitis. Microbiome, 2024. ⟨hal-04678119⟩