Skip to Main content Skip to Navigation
Journal articles

Active degradation of MarA controls coordination of its downstream targets

Abstract : Several key transcription factors have unusually short half-lives compared to other cellular proteins. Here, we explore the utility of active degradation in shaping how the multiple antibiotic resistance activator MarA coordinates its downstream targets. MarA controls a variety of stress response genes in Escherichia coli. We modify its half-life either by knocking down the protease that targets it via CRISPRi or by engineering MarA to protect it from degradation. Our experimental and analytical results indicate that active degradation can impact both the rate of coordination and the maximum coordination that downstream genes can achieve. In the context of multi-gene regulation, trade-offs between these properties show that perfect information fidelity and instantaneous coordination cannot coexist.
Complete list of metadata

Cited literature [48 references]  Display  Hide  Download
Contributor : Gestionnaire Hal-Upmc <>
Submitted on : Wednesday, January 16, 2019 - 12:01:22 PM
Last modification on : Thursday, December 10, 2020 - 12:37:58 PM


Publication funded by an institution



Nicholas Rossi, Thierry Mora, Aleksandra Walczak, Mary Dunlop. Active degradation of MarA controls coordination of its downstream targets. PLoS Computational Biology, Public Library of Science, 2018, 14 (12), pp.e1006634. ⟨10.1371/journal.pcbi.1006634⟩. ⟨hal-01983208⟩



Record views


Files downloads