An easy-to-implement combinatorial approach involving an activity-based assay for the discovery of a peptidyl copper complex mimicking superoxide dismutase

Amandine Vincent (1) , Jennifer Rodon Fores (1) , Elodie Tauziet (1) , Elodie Quévrain (2) , Ágnes Dancs (3) , Amandine Conte-Daban (4) , Anne-Sophie Bernard (1) , Philippe Pelupessy (5) , Koudedja Coulibaly (1) , Philippe Seksik (6) , Christelle Hureau (4) , Katalin Selmeczi (3) , Clotilde Policar (1) , Nicolas Delsuc (1)

1 LBM-E1 - Peptides, glycoconjugués et métaux en biologie
2 LBM-E1 - Peptides, glycoconjugués et métaux en biologie
3 L2CM - Laboratoire Lorrain de Chimie Moléculaire
4 LCC - Laboratoire de chimie de coordination
5 LBM-E3 - Structure et Dynamique des Biomolécules
6 CHU Saint-Antoine [AP-HP]

A combinatorial approach using a One-Bead-One-Compound method and a screening based on an SOD-activity assay was set up for the discovery of an efficient peptidyl copper complex. The complex exhibited good stability constants, suitable redox potentials and excellent intrinsic activity. This complex was further assayed in cells for its antioxidant properties and showed beneficial effect when cells were submitted to oxidative stress.

Domaines: Chimie de coordination; Chimie

Amandine Vincent, Jennifer Rodon Fores, Elodie Tauziet, Elodie Quévrain, Ágnes Dancs, et al.. An easy-to-implement combinatorial approach involving an activity-based assay for the discovery of a peptidyl copper complex mimicking superoxide dismutase. Chemical Communications, 2020, 56, pp.399-402. ⟨10.1039/C9CC07920C⟩. ⟨hal-02378888⟩