The emerging landscape of single-molecule protein sequencing technologies

Javier Antonio Alfaro (1) , Peggy Bohländer (2) , Mingjie Dai (3, 4) , Mike Filius (2) , Cecil J. Howard (5) , Xander F. Van Kooten (6) , Shilo Ohayon (6) , Adam Pomorski (2) , Sonja Schmid (7) , Aleksei Aksimentiev (8) , Eric V. Anslyn (5) , Georges Bedran (1) , Chan Cao (9) , Mauro Chinappi (10) , Etienne Coyaud (11, 12) , Cees Dekker (2) , Gunnar Dittmar (13, 14) , Nicholas Drachman (15) , Rienk Eelkema (2) , David Goodlett (1, 16) , Sébastien Hentz (17, 18) , Umesh Kalathiya (1) , Neil L. Kelleher (19) , Ryan T. Kelly (20) , Zvi Kelman (21) , Sung Hyun Kim (2) , Bernhard Kuster (22) , David Rodriguez-Larrea (23) , Stuart Lindsay (24) , Giovanni Maglia (25) , Edward M. Marcotte (5) , John P. Marino (21) , Christophe D. Masselon (26) , Michael Mayer (27) , Patroklos Samaras (22) , Kumar Sarthak (8) , Lusia Sepiashvili (28) , Derek Stein (15) , Meni Wanunu (29) , Mathias Wilhelm (22) , Peng Yin (3, 4) , Amit Meller (6) , Chirlmin Joo (2)

1 UG - University of Gdańsk
2 TU Delft - Delft University of Technology
3 Harvard University
4 HMS - Harvard Medical School [Boston]
5 University of Texas at Austin [Austin]
6 Technion - Israel Institute of Technology [Haifa]
7 WUR - Wageningen University and Research [Wageningen]
8 UIUC - University of Illinois at Urbana-Champaign [Urbana]
9 EPFL - Ecole Polytechnique Fédérale de Lausanne
10 Università degli Studi di Roma Tor Vergata [Roma, Italia] = University of Rome Tor Vergata [Rome, Italy] = Université de Rome Tor Vergata [Rome, Italie]
11 CHRU Lille - Centre Hospitalier Régional Universitaire [CHU Lille]
12 PRISM - Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U 1192
13 LIH - Luxembourg Institute of Health
14 uni.lu - Université du Luxembourg = University of Luxembourg = Universität Luxemburg
15 Brown University
16 UVIC - University of Victoria [Canada]
17 CEA-LETI - Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information
18 UGA - Université Grenoble Alpes
19 Northwestern University [Evanston]
20 BYU - Brigham Young University
21 IBBR - Institute for Bioscience and Biotechnology Research [Rockville, MD, États-Unis]
22 TUM - Technische Universität Munchen - Technical University Munich - Université Technique de Munich
23 UPV / EHU - Universidad del País Vasco [Espainia] / Euskal Herriko Unibertsitatea [España] = University of the Basque Country [Spain] = Université du pays basque [Espagne]
24 ASU - Arizona State University [Tempe]
25 University of Groningen [Groningen]
26 BGE - UMR S1038 - Laboratoire de Biologie à Grande Échelle
27 UNIFR - Université de Fribourg = University of Fribourg
28 University of Toronto
29 Northeastern University [Boston]

Single-cell profiling methods have had a profound impact on the understanding of cellular heterogeneity. While genomes and transcriptomes can be explored at the single-cell level, single-cell profiling of proteomes is not yet established. Here we describe new single-molecule protein sequencing and identification technologies alongside innovations in mass spectrometry that will eventually enable broad sequence coverage in single-cell profiling. These technologies will in turn facilitate biological discovery and open new avenues for ultrasensitive disease diagnostics.

Domaines: Sciences du Vivant [q-bio]

Javier Antonio Alfaro, Peggy Bohländer, Mingjie Dai, Mike Filius, Cecil J. Howard, et al.. The emerging landscape of single-molecule protein sequencing technologies. Nature Methods, 2021, 18 (6), pp.604--617. ⟨10.1038/s41592-021-01143-1⟩. ⟨hal-03605364⟩