Lens-free microscopy for 3D + time acquisitions of 3D cell culture

Anthony Berdeu (1) , Bastien Laperrousaz (2, 3) , Thomas Bordy (4) , Ondrej Mandula (5) , Sophie Morales (4) , Xavier X. Gidrol (3) , Nathalie Picollet-D’hahan (3) , Cédric Allier (4)

1 LISA - Laboratoire Imagerie et Systèmes d'Acquisition
2 Phys-ENS - Laboratoire de Physique de l'ENS Lyon
3 BIOMICS - Biomicrotechnologie et génomique fonctionnelle
4 CEA-LETI - Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information
5 NRS - Nanostructures et Rayonnement Synchrotron

Thanks to a novel three-dimensional imaging platform based on lens-free microscopy, it is possible to perform multi-angle acquisitions and holographic reconstructions of 3D cell cultures directly into the incubator. Being able of reconstructing volumes as large as ~5 mm$^3$ over a period of time covering several days, allows us to observe a broad range of migration strategies only present in 3D environment, whether it is single cell migration, collective migrations of cells and dispersal of cells. In addition we are able to distinguish new interesting phenomena, e.g. large-scale cell-to-matrix interactions (>1 mm), fusion of cell clusters into large aggregate (~10,000 µm$^2$) and conversely, total dissociation of cell clusters into clumps of migrating cells. This work on a novel 3D + time lens-free microscopy technique thus expands the repertoire of phenomena that can be studied within 3D cell cultures.

Domaines: Biologie cellulaire; Imagerie

Anthony Berdeu, Bastien Laperrousaz, Thomas Bordy, Ondrej Mandula, Sophie Morales, et al.. Lens-free microscopy for 3D + time acquisitions of 3D cell culture. Scientific Reports, 2018, 8 (1), ⟨10.1038/s41598-018-34253-6⟩. ⟨hal-02094236⟩