Nanobioengineering and Characterization of a Novel Estrogen Receptor Biosensor

Alexandre Berthier (1) , Céline Elie-Caille (2) , Eric Lesniewska (3) , Régis Delage-Mourroux (1) , Wilfrid Boireau (2)

1 E2SNC / ESTROGENES - Estrogènes, Expression génique et pathologies du Système Nerveux Central - UFC
2 FEMTO-ST - Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174)
3 ICB - Laboratoire Interdisciplinaire Carnot de Bourgogne

We constructed an original supramolecular assembly on a surface of sensor composed of an innovative combination of an engineered cytochrome b5 and a modified nucleic acid bound to a synthetic lipid hemimembrane. The protein/DNA block, called (PDNA) 2, was synthesized and purified before its immobilization onto a hybrid bilayer reconstituted on a gold surface. Surface plasmon resonance (SPR) and atomic force microscopy (AFM) were engaged in parallel on the same substrates in order to better understand dynamic events that occur at the surface of the biosensor. Good correlations were obtained in terms of specificity and reversibility. These findings allow us to present a first application of such biosensor in the study of the interaction processes between nuclear receptor and DNA

Mots clés: Nano-objects; molecular lego; AFM; SPR; Protein/DNA interaction

Domaines: Biophysique

Alexandre Berthier, Céline Elie-Caille, Eric Lesniewska, Régis Delage-Mourroux, Wilfrid Boireau. Nanobioengineering and Characterization of a Novel Estrogen Receptor Biosensor. Sensors, 2008, 8, pp.4413-4428. ⟨10.3390/s8074413⟩. ⟨hal-00347925⟩