The current landscape of coronavirus-host protein-protein interactions

Laure Perrin-Cocon (1) , Olivier Diaz (1) , Clémence Jacquemin (1) , Valentine Barthel (1) , Eva Ogire (1, 2) , Christophe Ramière (1, 3) , Patrice André (1) , Vincent Lotteau (1) , Pierre-Olivier Vidalain (1)

1 Biologie cellulaire des infections virales – Cell biology of viral infection [CIRI]
2 PIMIT - Processus Infectieux en Milieu Insulaire Tropical
3 Laboratoire de Virologie [HCL-Hôpital de la Croix Rousse]

In less than 20 years, three deadly coronaviruses, SARS‐CoV, MERS‐CoV and SARS‐CoV‐2, have emerged in human population causing hundreds to hundreds of thousands of deaths. Other coronaviruses are causing epizootic repre‐ senting a significant threat for both domestic and wild animals. Members of this viral family have the longest genome of all RNA viruses, and express up to 29 proteins establishing complex interactions with the host proteome. Deciphering these interactions is essential to identify cellular pathways hijacked by these viruses to replicate and escape innate immunity. Virus‐host interactions also provide key information to select targets for antiviral drug development. Here, we have manually curated the literature to assemble a unique dataset of 1311 coronavirus‐host protein–protein interactions. Functional enrichment and network‐based analyses showed coronavirus connections to RNA processing and translation, DNA damage and pathogen sensing, interferon production, and metabolic pathways. In particular, this global analysis pinpointed overlooked interactions with translation modulators (GIGYF2‐EIF4E2), components of the nuclear pore, proteins involved in mitochondria homeostasis (PHB, PHB2, STOML2), and methylation pathways (MAT2A/B). Finally, interactome data provided a rational for the antiviral activity of some drugs inhibiting coronavi‐ ruses replication. Altogether, this work describing the current landscape of coronavirus‐host interactions provides valuable hints for understanding the pathophysiology of coronavirus infections and developing effective antiviral therapies.

Mots clés: SARS-CoV-2; Coronavirus; Interactome; Virus-host interactions; Protein-protein interactions

Domaines: Sciences du Vivant [q-bio]; Microbiologie et Parasitologie; Virologie

Laure Perrin-Cocon, Olivier Diaz, Clémence Jacquemin, Valentine Barthel, Eva Ogire, et al.. The current landscape of coronavirus-host protein-protein interactions. Journal of Translational Medicine, 2020, ⟨10.1186/s12967-020-02480-z⟩. ⟨hal-02874650v3⟩