Vaginal Tampon Colonization by Staphylococcus aureus in Healthy Women

Myriam Chiaruzzi (1) , Alexia Barbry (1, 2) , Anaëlle Muggeo (2) , Anne Tristan (2, 3) , Isaline Jacquemond (3) , Cedric Badiou (3) , Laurence Cluzeau (1) , Sabine Bourdeau (3) , Thibaut Durand (2) , Astrid Engelmann (2) , Dorian Bosquet (2) , Michèle Bes (2, 3) , Claire Prigent-Combaret (4) , Jean Thioulouse (5) , Daniel Muller (4) , Gérard Lina (1, 2)

1 CIRI - Centre International de Recherche en Infectiologie
2 Hôpital de la Croix-Rousse [CHU - HCL]
3 CIRI-StaPath - Pathogénie des Staphylocoques – Staphylococcal Pathogenesis [CIRI]
4 LEM - Laboratoire d'Ecologie Microbienne - UMR 5557
5 Ecologie quantitative et évolutive des communautés [LBBE]

Tampons recovered from a cohort of 737 healthy women (median age, 32 years) were analyzed for the presence of Staphylococcus aureus. A total of 198 tampons (27%) were colonized by S. aureus, 28 (4%) by a strain producing toxic shock syndrome toxin 1 (TSST-1). S. aureus was detected more frequently in tampons that did not require an applicator for their insertion (74/233 [32%] versus 90/381 [24%]; odds ratio [OR] = 1.51 [95% confidence interval, 1.04 to 2.17]) and in women who used an intrauterine device for contraception (53/155 [34%] versus 145/572 [27%]; OR = 1.53 [95% confidence interval, 1.05 to 2.24]). The S. aureus strains isolated from tampons belonged to 22 different clonal complexes (CCs). The most prevalent CC was CC398 agr1 (n = 57 [27%]), a clone that does not produce superantigenic toxins, followed by CC30 agr3 (n = 27, 13%), producing TSST-1 (24/27 [89%]), the principal clone of S. aureus involved in menstrual toxic shock syndrome (MTSS). IMPORTANCE Menstrual toxic shock syndrome (MTSS) is an uncommon severe acute disease that occurs in healthy menstruating women colonized by TSST-1producing S. aureus who use intravaginal protection, such as tampons and menstrual cups. The catamenial product collected by the protection serves as a growth medium for S. aureus and allows TSST-1 production. Previous studies evaluated the prevalence of genital colonization by S. aureus by vaginal swabbing, but they did not examine tampon colonization. This study demonstrated a high prevalence of tampon colonization by S. aureus and the presence of the CC30 TSST-1 S. aureus clone responsible for MTSS in tampons from healthy women. The results support the vaginal carriage of this lineage in healthy women. In addition, the higher prevalence of S. aureus within tampons that do not require an applicator indicates a crucial role for handwashing before tampon handling to decrease the risk of tampon contamination.

Mots clés: Staphylococcus aureus; colonization; healthy women; menstruation; vagina

Domaines: Maladies infectieuses; Gynécologie et obstétrique; Santé publique et épidémiologie; Bactériologie

Myriam Chiaruzzi, Alexia Barbry, Anaëlle Muggeo, Anne Tristan, Isaline Jacquemond, et al.. Vaginal Tampon Colonization by Staphylococcus aureus in Healthy Women. Applied and Environmental Microbiology, 2020, 86 (18), pp.e01249-20. ⟨10.1128/AEM.01249-20⟩. ⟨hal-02989503v2⟩