Effects of a Lacticaseibacillus Mix on Behavioural, Biochemical, and Gut Microbial Outcomes of Male Mice following Chronic Restraint Stress

Vivien Letenneur (1) , Magali Monnoye (1) , Catherine Philippe (1) , Sophie Holowacz (2) , Sylvie Rabot (1) , Patricia Lepage (1, 3) , Elsa Jacouton (2) , Laurent Naudon (1, 4)

1 MICALIS - MICrobiologie de l'ALImentation au Service de la Santé
2 PiLeJe Laboratoire
3 ALIM-H - Département Alimentation Humaine - INRAE
4 CNRS - Centre National de la Recherche Scientifique

The effect of supplementation with Lactobacillus strains to prevent the consequences of chronic stress on anxiety in mouse strains sensitive to stress and the consequences on gut microbiota have been relatively unexplored. Thus, we administered a Lacticaseibacillus casei LA205 and Lacticaseibacillus paracasei LA903 mix to male BALB/cByJrj mice two weeks before and during 21-day chronic restraint stress (CRS) (non-stressed/solvent (NS-PBS), non-stressed/probiotics (NS-Probio), CRS/solvent (S-PBS), CRS/probiotics (S-Probio)). CRS resulted in lower body weight and coat state alteration, which were attenuated by the probiotic mix. S-Probio mice showed less stress-associated anxiety-like behaviours than their NS counterpart, while no difference was seen in PBS mice. Serum corticosterone levels were significantly higher in the S-Probio group than in other groups. In the hippocampus, mRNA expression of dopamine and serotonin transporters was lower in S-Probio than in S-PBS mice. Few differences in bacterial genera proportions were detected, with a lower relative abundance of Alistipes in S-Probio vs. S-PBS. CRS was accompanied by a decrease in the proportion of caecal acetate in S-PBS mice vs. NS-PBS, but not in the intervention groups. These data show that the probiotic mix could contribute to better coping with chronic stress, although the precise bacterial mechanism is still under investigation.

Mots clés: psychobiotics microbiota-gut-brain-axis chronic stress anxiety behaviour; psychobiotics; microbiota-gut-brain-axis; chronic stress; anxiety behaviour

Domaines: Sciences du Vivant [q-bio]

Vivien Letenneur, Magali Monnoye, Catherine Philippe, Sophie Holowacz, Sylvie Rabot, et al.. Effects of a Lacticaseibacillus Mix on Behavioural, Biochemical, and Gut Microbial Outcomes of Male Mice following Chronic Restraint Stress. Nutrients, 2023, 15 (21), pp. 4635. ⟨10.3390/nu15214635⟩. ⟨hal-04282336v2⟩