Arabidopsis proteins with a transposon-related domain act in gene silencing

Yoko Ikeda (1, 2) , Thierry Pélissier (1) , Pierre Bourguet (1) , Claude Becker (3) , Marie-Noelle Pouch-Pelissier (1) , Romain Pogorelcnik (1) , Magdalena Weingartner (4) , Detlef Weigel (3) , Jean-Marc Deragon (5) , Olivier Mathieu (1)

1 GReD - Génétique, Reproduction et Développement
2 Institute of Plant Science and Resources
3 GMI - Gregor Mendel Institute of Molecular Plant Biology
4 MPI-MP - Max-Planck-Institut für Molekulare Pflanzenphysiologie
5 LGDP - Laboratoire Génome et développement des plantes

Transposable elements (TEs) are prevalent in most eukaryotes, and host genomes have devised silencing strategies to rein in TE activity. One of these, transcriptional silencing, is generally associated with DNA methylation and short interfering RNAs. Here we show that the Arabidopsis genes MAIL1 and MAIN define an alternative silencing pathway independent of DNA methylation and short interfering RNAs. Mutants for MAIL1 or MAIN exhibit release of silencing and appear to show impaired condensation of pericentromeric heterochromatin. Phylogenetic analysis suggests not only that MAIL1 and MAIN encode a retrotransposon related plant mobile domain, but also that host plant mobile domains were captured by DNA transposons during plant evolution. Our results reveal a role for Arabidopsis proteins with a transposon-related domain in gene silencing.

Domaines: Sciences du Vivant [q-bio]

Yoko Ikeda, Thierry Pélissier, Pierre Bourguet, Claude Becker, Marie-Noelle Pouch-Pelissier, et al.. Arabidopsis proteins with a transposon-related domain act in gene silencing. Nature Communications, 2017, 8, ⟨10.1038/ncomms15122⟩. ⟨hal-01917158⟩